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lentiviral constructs  (Addgene inc)


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    Addgene inc lentiviral constructs
    Lentiviral Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+lentiviral+plasmids/pLV-EF1-Cre-PGK-Puro+(Plasmid+%23108543)/pm41689802-397-8-12
    Average 93 stars, based on 7 article reviews
    lentiviral constructs - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Rejuvenation of the Aged Stem Cell Niche: Signal Transduction and Reversing the Decline of Adult Hippocampal Neurogenesis and Myogenesis
    Article Snippet: .. Cre lentiviral plasmids were obtained (Addgene http://www.addgene.org/20781/ and http://www.addgene.org/27546/), packaged, and purified as previously described (Yu & Schaffer 2006; Peltier & Schaffer 2010). .. For BrdU in vivo labeling of mitotic cells, mice were intraperitoneally injected with BrdU (50 mg/kg of body weight, Sigma Aldrich) dissolved in saline to label mitotic cells, as previously described (Lai et al. 2003).

    Article Title: Age-Associated Increase in BMP Signaling inhibits Hippocampal Neurogenesis
    Article Snippet: .. Cre lentiviral plasmids were obtained (Addgene http://www.addgene.org/20781/ and http://www.addgene.org/27546/ ), packaged, and purified as previously described [ 60 , 61 ]. ..



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    Primary neurons were infected with lentivirus encoding Ftl1 or control at 8 days in vitro (DIV8) and analyzed at DIV18. ( a ) Illustration showing the coverslip regions used for neurite analysis of primary neurons following lentiviral-mediated overexpression of Ftl1 (Ftl1 OE) or control. ( b ) Representative images of GFP-positive neurons used as a redout for lentiviral infection for both Ftl1 OE and control conditions. ( c ) Representative Western blot and quantification of FTL1 protein levels N = 8 replicates/condition (p-value < 0.0001). ( d ) Quantification of cell death in primary neurons following viral-mediated overexpression of Ftl1 by LDH assay. N = 5 positive control, N = 5 control and N = 5 Ftl1 OE replicates/condition (p-value < 0.0001). ( e ) Representative images and quantification of DAPI-positive nuclei of Ftl1 OE and control neurons at DIV18. N = 5 controls and N = 5 Ftl1 OE replicates/condition (p-value = 0.8956). Data shown as mean±s.e.m.; ****P < 0.001, two-tailed t-test ( c,d,e ).

    Journal: Nature Aging

    Article Title: Targeting iron-associated protein Ftl1 in the brain of old mice improves age-related cognitive impairment

    doi: 10.1038/s43587-025-00940-z

    Figure Lengend Snippet: Primary neurons were infected with lentivirus encoding Ftl1 or control at 8 days in vitro (DIV8) and analyzed at DIV18. ( a ) Illustration showing the coverslip regions used for neurite analysis of primary neurons following lentiviral-mediated overexpression of Ftl1 (Ftl1 OE) or control. ( b ) Representative images of GFP-positive neurons used as a redout for lentiviral infection for both Ftl1 OE and control conditions. ( c ) Representative Western blot and quantification of FTL1 protein levels N = 8 replicates/condition (p-value < 0.0001). ( d ) Quantification of cell death in primary neurons following viral-mediated overexpression of Ftl1 by LDH assay. N = 5 positive control, N = 5 control and N = 5 Ftl1 OE replicates/condition (p-value < 0.0001). ( e ) Representative images and quantification of DAPI-positive nuclei of Ftl1 OE and control neurons at DIV18. N = 5 controls and N = 5 Ftl1 OE replicates/condition (p-value = 0.8956). Data shown as mean±s.e.m.; ****P < 0.001, two-tailed t-test ( c,d,e ).

    Article Snippet: Validated gRNA sequences were subcloned into a Syn1 -Cre lentiviral vector (Addgene, no. 68841; ref. ) using PacI and NheI/XbaI.

    Techniques: Infection, Control, In Vitro, Over Expression, Western Blot, Lactate Dehydrogenase Assay, Positive Control, Two Tailed Test

    Primary neurons were infected with lentivirus encoding shRNA targeting Ftl1 or control at 8 days in vitro (DIV8) and analyzed at DIV18. ( a ) Illustration showing the coverslip regions used for neurite analysis of primary neurons following lentiviral-mediated Ftl1 knockdown (Ftl1 KD) or control. ( b ) Representative images of GFP-positive neurons used as a redout for lentiviral infection for both Ftl1 KD and control conditions. ( c ) Representative Western blot and quantification of FTL1 protein levels. N = 8 replicates/condition N= (p-value < 0.0001) ( d ) Quantification of cell death in primary neurons following viral-mediated overexpression of Ftl1 by LDH assay. ( d ) Representative images and quantification of DAPI-positive nuclei of Ftl1 KD and control neurons at DIV18. N = 5 positive control, N = 5 control and N = 5 Ftl1 KD replicates/condition (p-value < 0.0001). ( e ) Representative images and quantification of DAPI-positive nuclei of Ftl1 KD and control neurons at DIV18. N = 5 controls and N = 5 Ftl1 KD replicates/condition (p-value = 0.2130). Data shown as mean±s.e.m.; ****P < 0.001, two-tailed t-test ( c,d,e ).

    Journal: Nature Aging

    Article Title: Targeting iron-associated protein Ftl1 in the brain of old mice improves age-related cognitive impairment

    doi: 10.1038/s43587-025-00940-z

    Figure Lengend Snippet: Primary neurons were infected with lentivirus encoding shRNA targeting Ftl1 or control at 8 days in vitro (DIV8) and analyzed at DIV18. ( a ) Illustration showing the coverslip regions used for neurite analysis of primary neurons following lentiviral-mediated Ftl1 knockdown (Ftl1 KD) or control. ( b ) Representative images of GFP-positive neurons used as a redout for lentiviral infection for both Ftl1 KD and control conditions. ( c ) Representative Western blot and quantification of FTL1 protein levels. N = 8 replicates/condition N= (p-value < 0.0001) ( d ) Quantification of cell death in primary neurons following viral-mediated overexpression of Ftl1 by LDH assay. ( d ) Representative images and quantification of DAPI-positive nuclei of Ftl1 KD and control neurons at DIV18. N = 5 positive control, N = 5 control and N = 5 Ftl1 KD replicates/condition (p-value < 0.0001). ( e ) Representative images and quantification of DAPI-positive nuclei of Ftl1 KD and control neurons at DIV18. N = 5 controls and N = 5 Ftl1 KD replicates/condition (p-value = 0.2130). Data shown as mean±s.e.m.; ****P < 0.001, two-tailed t-test ( c,d,e ).

    Article Snippet: Validated gRNA sequences were subcloned into a Syn1 -Cre lentiviral vector (Addgene, no. 68841; ref. ) using PacI and NheI/XbaI.

    Techniques: Infection, shRNA, Control, In Vitro, Knockdown, Western Blot, Over Expression, Lactate Dehydrogenase Assay, Positive Control, Two Tailed Test

    Primary neurons were infected with lentivirus encoding Ftl1 or control at 8 days in vitro (DIV8) subsequently treated with NADH (200uM) for 5 consecutive days starting at DIV14 and analyzed at DIV18. ( a ) Schematic illustrating experimental timeline for lentiviral-mediated overexpression of Ftl1 (Ftl1 OE) or control and NADH treatment in primary neurons. ( b ) Quantification of cell death in primary neurons following viral-mediated overexpression of Ftl1 and NADH treatment by Lactate Dyhydrogenase (LDH) assay. Each data point represents the average of 3 replicates. Data shown as mean±s.e.m, two-way ANOVA followed by Tukey’s post hoc t-test.

    Journal: Nature Aging

    Article Title: Targeting iron-associated protein Ftl1 in the brain of old mice improves age-related cognitive impairment

    doi: 10.1038/s43587-025-00940-z

    Figure Lengend Snippet: Primary neurons were infected with lentivirus encoding Ftl1 or control at 8 days in vitro (DIV8) subsequently treated with NADH (200uM) for 5 consecutive days starting at DIV14 and analyzed at DIV18. ( a ) Schematic illustrating experimental timeline for lentiviral-mediated overexpression of Ftl1 (Ftl1 OE) or control and NADH treatment in primary neurons. ( b ) Quantification of cell death in primary neurons following viral-mediated overexpression of Ftl1 and NADH treatment by Lactate Dyhydrogenase (LDH) assay. Each data point represents the average of 3 replicates. Data shown as mean±s.e.m, two-way ANOVA followed by Tukey’s post hoc t-test.

    Article Snippet: Validated gRNA sequences were subcloned into a Syn1 -Cre lentiviral vector (Addgene, no. 68841; ref. ) using PacI and NheI/XbaI.

    Techniques: Infection, Control, In Vitro, Over Expression, Lactate Dehydrogenase Assay